This course provides a general overview of the use of fixatives in cellular pathology, outlining the changes that occur in cells and tissues when exposed to cold-ischaemia and the need for immediate tissue fixation. The content covers the evolution of fixation and fixatives, and the chemical changes produced in tissue proteins by exposure to additive and coagulative fixatives. There is also guidance on how different fixatives effect the quality of haematoxylin and eosin staining
