Diagnostic Direct Immunofluorescence (DIF) is used in histopathology and immunology centers, mainly for autoimmune bullous diseases in dermatology and mucosal specimens. It’s also valuable in renal pathology and occasionally in nerve and muscle tests. While DIF is used alongside other histological techniques, it’s the gold standard for diagnosing conditions like pemphigus and pemphigoid. mages from tissue sections prepared for DIF should highlight key features that aid diagnosis, offering a clear representation of the fluorescence findings. The images must be in focus with sufficient contrast to clearly display the immunofluorescence features.

Rationale

The DIF scheme evaluates 4 archival positive fluorescence digital images. The specimen types and fluorescent markers required for submission are not specified and Participants are permitted to submit any representative image showing positive staining. The magnification of each image should be appropriate to its purpose, the purpose being an overview of the biopsy as a whole or demonstration of one specific diagnostic feature.

Submission

4 positive fluorescence images, as indicate on the delivery letter, regardless of the number of cases. Participants must submit at least 4 distinct positive conjugate examples that represent their in-house repertoire.

Results Reporting

• Individualised Results Report
• Generic Image Report
• Generic Best Method Report
• Annual Report